Ryan Tewhey

Ryan Tewhey is a postdoctoral fellow in the Sabeti lab. His primary focus is on the development of high-throughput screening systems to understand the functional impact of non-coding polymorphisms. He is currently applying the massively parallel reporter assay (MPRA) to identify causal alleles driving positive selection signals and loci identified from genome wide association studies. His broad interests lie in the application of novel technologies to answer questions related to human health and disease. His time is evenly divided between wet and dry lab approaches.

Ryan completed a dual major in Cellular Biology and Biochemistry at the University of Maine, Orono and then worked for two years at the Broad Institute assisting with the first genome wide association studies for type II diabetes. He received his PhD from the Division of Biological Science at University of California, San Diego in 2012 while carrying out work at The Scripps Research Institute. Here he developed technologies for high-throughput sequencing studies and used the technologies to study human complex diseases as well as microbial pathogenesis. He was named to Forbes Magazine's 30 under 30 in science in 2011 and has conducted research at the Genome Institute of Singapore as a recipient of a NSF/A-STAR EAPSI fellowship. He is currently a supported by an NIH Pathway to Independence award.


Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples.
Genome Biology. 2014 Nov 18
Matranga CB, Andersen KG, Winnicki S, Busby M, Gladden AD, Tewhey R, Stremlau M, Berlin A, Gire SK, England E, Moses LM, Mikkelsen TS, Odia I, Ehiane PE, Folarin O, Goba A, Kahn S, Grant DS, Honko A, Hensley L, Happi C, Garry RF, Malboeuf CM, Birren BW, Gnirke A, Levin JZ, Sabeti PC.

Mechanisms of linezolid resistance among coagulase-negative staphylococci determined by whole-genome.
sequencing. MBio. 2014 May 13.
Tewhey R, Gu B, Kelesidis T, Charlton C, Bobenchik A, Hindler J, Schork NJ, Humphries RM.

Evidence for the role of EPHX2 gene variants in anorexia nervosa.
Mol Psychiatry. 2014 Jun.
Scott-Van Zeeland AA, Bloss CS, Tewhey R, Bansal V, Torkamani A, Libiger O, Duvvuri V, Wineinger N, Galvez L, Darst BF, Smith EN, Carson A, Pham P, Phillips T, Villarasa N, Tisch R, Zhang G, Levy S, Murray S, Chen W, Srinivasan S, Berenson G, Brandt H, Crawford S, Crow S, Fichter MM, Halmi KA, Johnson C, Kaplan AS, La Via M, Mitchell JE, Strober M, Rotondo A, Treasure J, Woodside DB, Bulik CM, Keel P, Klump KL, Lilenfeld L, Plotnicov K, Topol EJ, Shih PB, Magistretti P, Bergen AW, Berrettini W, Kaye W, Schork NJ.

Clinical implications of human population differences in genome-wide rates of functional genotypes.
Front Genet. 2012.
Torkamani A, Pham P, Libiger O, Bansal V, Zhang G, Scott-Van Zeeland AA,
Tewhey R, Topol EJ, Schork NJ.

Genetic structure of community acquired methicillin-resistant Staphylococcus aureus USA300.
BMC Genomics. 2012.
Tewhey R, Cannavino CR, Leake JA, Bansal V, Topol EJ, Torkamani A, Bradley JS, Schork NJ.

Whole genome sequencing analysis of Plasmodium vivax using whole genome capture.
BMC Genomics. 2012.
Bright AT, Tewhey R, Abeles S, Chuquiyauri R, Llanos-Cuentas A, Ferreira MU, Schork NJ, Vinetz JM, Winzeler EA.

The importance of phase information for human genomics.
Nat Rev Genet. 2011.
Tewhey R, Bansal V, Torkamani A, Topol EJ, Schork NJ.

Microdroplet-based PCR enrichment for large-scale targeted sequencing.
Nat Biotechnol. 2009.
Tewhey R, Warner JB, Nakano M, Libby B, Medkova M, David PH, Kotsopoulos SK, Samuels ML, Hutchison JB, Larson JW, Topol EJ, Weiner MP, Harismendy O, Olson J, Link DR, Frazer KA.

Enrichment of sequencing targets from the human genome by solution hybridization.
Genome Biol. 2009.
Tewhey R, Nakano M, Wang X, Pabón-Peña C, Novak B, Giuffre A, Lin E, Happe S, Roberts DN, LeProust EM, Topol EJ, Harismendy O, Frazer KA.

Common missense variant in the glucokinase regulatory protein gene is associated with increased plasma triglyceride and C-reactive protein but lower fasting glucose concentrations.
Diabetes. 2008 Nov.
Orho-Melander M, Melander O, Guiducci C, Perez-Martinez P, Corella D, Roos C, Tewhey R, Rieder MJ, Hall J, Abecasis G, Tai ES, Welch C, Arnett DK, Lyssenko V, Lindholm E, Saxena R, de Bakker PI, Burtt N, Voight BF, Hirschhorn JN, Tucker KL, Hedner T, Tuomi T, Isomaa B, Eriksson KF, Taskinen MR, Wahlstrand B, Hughes TE, Parnell LD, Lai CQ, Berglund G, Peltonen L, Vartiainen E, Jousilahti P, Havulinna AS, Salomaa V, Nilsson P, Groop L, Altshuler D, Ordovas JM, Kathiresan S.

Genome-wide association analysis identifies loci for type 2 diabetes and triglyceride levels.
Science. 2007
Diabetes Genetics Initiative of Broad Institute of Harvard and MIT, Lund University, and Novartis Institutes of BioMedical Research1, Saxena R, Voight BF, Lyssenko V, Burtt NP, de Bakker PI, Chen H, Roix JJ, Kathiresan S, Hirschhorn JN, Daly MJ, Hughes TE, Groop L, Altshuler D, Almgren P, Florez JC, Meyer J, Ardlie K, Bengtsson Boström K, Isomaa B, Lettre G, Lindblad U, Lyon HN, Melander O, Newton-Cheh C, Nilsson P, Orho-Melander M, Råstam L, Speliotes EK, Taskinen MR, Tuomi T, Guiducci C, Berglund A, Carlson J, Gianniny L, Hackett R, Hall L, Holmkvist J, Laurila E, Sjögren M, Sterner M, Surti A, Svensson M, Svensson M, Tewhey R, Blumenstiel B, Parkin M, Defelice M, Barry R, Brodeur W, Camarata J, Chia N, Fava M, Gibbons J, Handsaker B, Healy C, Nguyen K, Gates C, Sougnez C, Gage D, Nizzari M, Gabriel SB, Chirn GW, Ma Q, Parikh H, Richardson D, Ricke D, Purcell S.

For a complete list of publications: (http://www.ncbi.nlm.nih.gov/pubmed/?term=Tewhey+R)

rtewhey |at| broadinstitute.org